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Reversible alterations in the inner mitochondrial membrane associated with oxygen depletion during isolation of liver.
Isolation of liver with Ficoll-sucrose density gradient results in an immediate but reversible drop of oxygen in the medium that is probably due to the sudden increase in membrane area. About 30% of the initial respiration of liver is lost, immediately after the isolation. Measurements of reduced and oxidized glutathione levels in intact mitochondria showed that the reserve thiol pairs redox potential 6add127376 gerbal
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