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This study describes methods for the ultrastructural localization of the glial fibrillary acidic protein (GFA) within nervous tissue. A hydrated freeze-substitution technique was utilized to attenuate antigen-antibody reactions for GFA. Immuno-gold labeling of GFA in the nervous system revealed no visible reaction between GFA antibodies and proteins adsorbed to metal phase http://pariswilton.com/xforce-keygen-32bits-or-64bits-version-flare-2014-free-download-fix/
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